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Views: (1386) Date: (02-06-12) Time: (00:28:52) |
Description:
Antigens (biomarkers) have been microarrayed on a specially polished and treated glass microscope slide using US Patent 6,101,946. This slide contains biomarkers which can be incubated with either diluted patient serum or plasma. Patient serum IgG will bind to recognized biomarkers printed on the microarray during the first incubation. After washing away unbound IgGs, anti-human IgG-secondary antibody conjugated to a fluorescent dye is added to the microarrays which bind to the IgG-biomarker conjugate. Unbound IgGsecondary antibody is removed by washing. The slides are dried by Microarray Centrifuge and are then ready for scanning and quantification using a high resolution microarray laser scanner (Innoscan 710) and software (Mapix) package.