Protein import into nuclei is mediated by the nuclear pore complex (NPC) and by cellular factors. To structurally characterize this process, nuclear import of gold-labeled nucleoplasmin was followed b...
We have characterized several intermediates in the mitochondrial import stimulation factor (MSF)-dependent import into mammalian mitochondria of a matrix-targeted precursor, preadrenodoxin (pAd). In t...
The heterotrimeric Toc core complex of the chloroplast protein import apparatus contains two GTPases, Toc159 and Toc34, together with the protein-conducting channel Toc75. Toc159 and Toc34 are exposed...
We have previously reported that the precursor of rat liver mitochondrial malate dehydrogenase, synthesized in vitro, is about 1,500 to 2,000 Mr larger than the mature enzyme and can be processed to t...
Mas20p and Mas70p are integral proteins of the yeast mitochondrial outer membrane that appear to function as receptors for precursor proteins imported from the cytosol. Loss of either receptor alone d...
Extramitochondrial pools of mitochondrial precursor polypeptides can be detected in yeast spheroplasts which had been pulse-labeled for 3 min in the absence of inhibitors or for 10 min in the presence...
In order to identify functionally important amino acid residues in the chloroplast protein import machinery, chloroplasts were preincubated with amino-acid-modifying reagents and then allowed to impor...