Quantitation of model digestive mixtures by 13C NMR

Abstract:  Quantitation of model digestive mixtures by 13C NMR. Wang D, Hadipour NL, Jerlin EA, Stark RE. Department of Chemistry, College of Staten Island, City University of New York 10301. 13C nuclear magnetic resonance (NMR) spectra were obtained at 50.3 and 100.5 MHz for methanolic and aqueous mixtures of sodium taurocholate, 1-monocapryloyl-rac- glycerol, and caprylic acid. Distortionless Enhancement by Polarization Transfer (DEPT) was used to improve spectral sensitivity and resolution, and to generate calibration curves for quantitative determinations of each lipid in methanol. Alternatively, the heights for nonoverlapping peaks in a 13C NMR spectrum acquired with inverse-gated decoupling provide reliable quantitative estimates for each component of the mixture, particularly when the data are obtained in methanol. These experiments also demonstrate the feasibility of detailed NMR structural investigations in model systems for glyceride digestion. PMID: 1569390 [PubMed - indexed for MEDLINE]