New functional assignment of the carotenogenic genes crtB and crtE with constructs of these genes from Erwinia species


     Related Videos

     Related Hubpages

    Field: Medicine  DOWNLOAD  BOOKMARK REPORT
    •  Doc. Url:    Embed Code: 

    • PubMed  status
      (0) (0 Votes)
      		Views: (1044)   Date: (13-03-09)   Pages: ()

    • Author:  Sandmann G  Misawa N.  

    • Abstract:  New functional assignment of the carotenogenic genes crtB and crtE with constructs of these genes from Erwinia species. Sandmann G, Misawa N. Lehrstuhl f?r Physiologie und Biochemie der Pflanzen, Universit?t Konstanz, F.R.G. The role of carotenoid genes crtB and crtE has been functionally assigned. These genes were cloned from Erwinia into Escherichia coli or Agrobacterium tumefaciens. Their functions were elucidated by assaying early isoprenoid enzymes involved in phytoene formation. In vitro reactions from extracts of E. coli carrying the crtE gene or a complete carotenogenic gene cluster in which crtB was deleted showed an elevated conversion of farnesyl pyrophosphate (FPP) into geranylgeranyl pyrophosphate (GGPP). These results strongly indicate that the crtE gene encodes GGPP synthase. Introduction of the crtB gene into A. tumefaciens led to the conversion of GGPP into phytoene. This activity was absent in similar transformants with the crtE gene. Thus, the crtB gene probably encodes phytoene synthase, which was further supported by demonstration that phytoene accumulated in E. coli harboring both the crtB and crtE genes. PMID: 1555761 [PubMed - indexed for MEDLINE]

         Related Documents

           Related Groups

             Related Science News

               More on Sciencestage

               Answers

               News
          • New Maldives president says willing to call early election
            DUBAI (Reuters) - The Maldives' new leader said a presidential election could be held before the scheduled date of October 2013 provided conditions were right for a free and peaceful poll, after his predecessor quit last week amid a dramatic police mutiny. Mohamed Waheed Hussain Manik, previously vice president, took over after the February 7 resignation of Mohamed Nasheed, the Maldives' first ...
          • New Zealand Dollar Slides as Asian Stocks Drop; Aussie Erases Earlier Gain
            New Zealand’s dollar declined against all of its 16 major counterparts as Asian stocks fell, damping demand for higher-yielding assets.
          • GQ Names "The Ten Best New Restaurants in America" for 2012
            NEW YORK, Feb. 16, 2012 /PRNewswire/ -- GQ has announced " The Ten Best New Restaurants in America " for 2012. The annual feature appears in the March issue of the magazine, available on newsstands ...
          • New malaria method could boost drug production
            German scientists have developed a new way to make a key malaria drug that they say could easily quadruple production and drop the price significantly, increasing the availability of treatment for a disease that kills hundreds of thousands every year.
          • New Mexico beats No. 13 SDSU 77-67
            New Mexico came into No. 13 San Diego State's loud, crazy arena and made a bold statement of their own. Kendall Williams scored 21 points and Drew Gordon had 17 points and 17 rebounds as the Lobos stunned the Aztecs 77-67 on Wednesday night to move into sole possession of first place in the Mountain West Conference.
          • A new NBA star, Jeremy Lin becomes a bankable name
            NEW YORK (Reuters) - Less than two weeks ago anyone looking to buy a ticket to watch the New York Knicks play the Dallas Mavericks this weekend could expect to pay around $270. Today, the same ticket would cost about twice that. Call it Linflation. Jeremy Lin's surprising basketball heroics have made the 23-year-old point guard a sports sensation and media darling nearly overnight, and there are ...
          • New ETF Drills Into Fracking Space
            NEW YORK (TheStreet) -- This week Van Eck launched the Market Vectors Unconventional Oil & Gas ETF . The ticker symbol gives an indication that fracking (hydraulic fracturing) of shale oil and shale gas is a key theme in the fund. Other so-called unconventional extraction methods targeted by the fund include coal seam gas, coal bed methane and even oilsands. The fund has 44 constituent holdings ...
          • New York state owes poor schools $5.5 billion: advocates
            (Reuters) - Education advocates on Wednesday pushed New York State to increase funding for schools in impoverished areas in the budget for the new fiscal year, saying those schools are owed $5.5 billion under a 2007 court ruling. The Education Law Center, which absorbed the advocate group that had won the historic school funding lawsuit, did not specify how much extra funding it is seeking in ...
          • New Hope supporters turn out for rally against possible charter loss
            If New Hope Academy were to close, Olga Ortiz would move out of York City. The mother of a fifth-grade New Hope student and a New Hope graduate said she doesn't want her son, Quazar Ortiz, to return to the York City School District.
          • Celebrities arriving in New Orleans for Mardi Gras
            Actress Patricia Clarkson received a brass band reception when she arrived Wednesday in New Orleans, setting the stage for the all-women Krewe of Muses parade to roll Thursday night.

               Related on Wikipedia




























           

          Powered free by PHPmotion