LamB maltoporin of Salmonella typhimurium isolation purification and comparison of sugar binding with LamB of Escherichia coli


     Related Videos
Tracking system is dam good news for salmon
Sea lice may be driving wild salmon to extinction
The Holy Cow and the Lamb of God in Hinduism and Judaism Pt 1
The Holy Cow and the Lamb of God in Hinduism and Judaism Pt 2
Heaven - The Lion and the Lamb - YouTube

     Related Hubpages

  •  Doc. Url:    Embed Code: 

  • PubMed  status
    (0) (0 Votes)
    Views: (1031)   Date: (27-01-09)   Pages: ()
  • Author:  Sch?lein K  Benz R.  

  • Abstract:  LamB (maltoporin) of Salmonella typhimurium: isolation, purification and comparison of sugar binding with LamB of Escherichia coli. Sch?lein K, Benz R. Lehrstuhl f?r Biotechnologie, Universit?t W?rzburg, FRG. LamB (maltoporin) of Salmonella typhimurium was found to be more strongly associated with the murein than OmpF. It was purified in one step using a hydroxyapatite (HTP) column. Reconstitution of the pure protein with lipid bilayer membrane showed that LamB of S. typhimurium formed small ion-permeable channels with a single channel conductance of about 90 pS in 1 M KCl and some preference for cations over anions. The conductance concentration curve was linear, which suggested that LamB of S. typhimurium does not contain any binding site for ions. Pore conductance was completely inhibited by the addition of 20 mM maltotriose. Titration of the LamB-induced membrane conductance with different sugars, including all members of the maltooligosaccharide series up to seven glucose residues, suggested that the channel contains, like LamB (maltoporin) of Escherichia coli, a binding site for sugars. The binding constant of sugars of the maltooligosaccharide series increased with increasing number of glucose residues up to five (saturated). Small sugars had a higher stability constant for sugar binding relative to LamB of E. coli. The advantage of a binding site inside a specific porin for the permeation of solutes is discussed with respect to the properties of a general diffusion porin. PMID: 1693746 [PubMed - indexed for MEDLINE]

     Related Documents

         Related Groups

           Related Science News

             More on Sciencestage

               Answers

                 News

                   Related on Youtube Results
            • Guest Speaker Brian Lamb part 1
              Guest Speaker Brian Lamb part 1 From: RotherhamMBC Views: 0 0 ratings Time: 14:39 More in Entertainment
            • Guest speaker Brian Lamb part 2
              Guest speaker Brian Lamb part 2 From: RotherhamMBC Views: 0 0 ratings Time: 13:57 More in Entertainment
            • The Binding Of Isaac: Wrath Of The Lamb #140
              The Binding Of Isaac: Wrath Of The Lamb #140 The Binding Of Isaac Playlist: http://bit.ly/1r9UGsl ▻ Videospiele günstig kaufen: http://bit.ly/14J2oiZ ▻ Livestreams: http://www.twitch.tv/wolverous ▻ Let's Play Übersicht: http://bi... From: TheWolverous Views: 0 0 ratings Time: 15:45 More in Gaming
            • Speaker Brian Lamb part 3
              Speaker Brian Lamb part 3 From: RotherhamMBC Views: 0 0 ratings Time: 12:02 More in Entertainment
            • Đồ rê mí - Nhạc cho thiếu nhi - Mary had a little lamb
              Đồ rê mí - Nhạc cho thiếu nhi - Mary had a little lamb Kênh thiếu nhi đang được xây dựng và đi vào hoạt động bước đầu với rất nhiều nội dung đa dạng như chuyên mục chuyện cổ tích, thế giới động... From: Kênh Thiếu Nhi Views: 0 0 ratings Time: 03:09 More in People & Blogs
            • @9am Worthy is the Lamb
              @9am Worthy is the Lamb Surprise # (on the spot,hehe) From: Djessa Cantorne Views: 0 0 ratings Time: 04:34 More in People & Blogs
            • Worthy is the Lamb by Sinach
              Worthy is the Lamb by Sinach From: africagospelmusicvideos two Views: 0 0 ratings Time: 04:53 More in People & Blogs
            • Mary had a little lamb Piano Tutorial | 4 kidz
              Mary had a little lamb Piano Tutorial | 4 kidz Learn a piano song easy and fast. From: Mythsie Watty Views: 0 0 ratings Time: 00:16 More in People & Blogs
            • Marry Had A Little Lamb Karaoke Version With Lyrics Animated English Nursery Rhymes For Ki
              Marry Had A Little Lamb Karaoke Version With Lyrics Animated English Nursery Rhymes For Ki Marry Had A Little Lamb Karaoke Version With Lyrics Animated English Nursery Rhymes For Kids Subscribe here: MAGIC BOX ANIMATION PROUDLY PRESENTS Animated English Nursery Rhymes ... From: Angela Willis Views: 0 0 ratings Time: 02:16 More in People & Blogs
            • [MMD] Lamb. [Happy Halloween!]
              [MMD] Lamb. [Happy Halloween!] Happy Halloween everyone! I've been waiting for a month to finally post this ( ≧Д≦)/ I hope everyone likes it. I do. As always, watch in HD! Motion: Lamb. - sm22498017 Cam: kimberlityan... From: Shelby W. Views: 1 0 ratings Time: 03:56 More in Film & Animation
            • Worthy Is The Lamb Brooklyn Tabernacle Choir
              Worthy Is The Lamb Brooklyn Tabernacle Choir Description. From: Frequence VIE Views: 0 0 ratings Time: 05:38 More in Entertainment
            • Holy Lamb Organics Wool and Cotton Body Pillows
              Holy Lamb Organics Wool and Cotton Body Pillows Holy Lamb Organics Wool and Cotton Body Pillows http://www.amazon.com/gp/product/B007QWYADS?tag=ndolohokibeut-20. From: Petra Božić Views: 0 0 ratings Time: 00:55 More in Science & Technology
            • NALLARUCHI Episode 33 Lamb Shank with redwine sauce; Safron Jilebi (full) 26th October
              NALLARUCHI Episode 33 Lamb Shank with redwine sauce; Safron Jilebi (full) 26th October Lamb Shank with red wine sauce; Safron Jilebi by Chef Thomas shankan Antony, Hotel Medos, International, Chalakkudy (full) NALLARUCHI Episode 33 26th October 2014. From: Nalla Ruchi Views: 0 0 ratings Time: 23:15 More in People & Blogs
            • Sesame Street Don Music Rewrites Mary Had A Little Lamb
              Sesame Street Don Music Rewrites Mary Had A Little Lamb Sesame, Street, David, Muppets, Northern, Calloway, Gina, Allision, Barnett, Jerry, Nelson, Richard, Hunt, Kevin, Clash, rock, music, doo-wop, stars,sesame street,sesame street songs,sesame... From: Animation World Network Views: 0 0 ratings Time: 04:59 More in Film & Animation
            • Lamb Of God - Blacken The Cursed Sun
              Lamb Of God - Blacken The Cursed Sun Aaaaand here's another one, Lamb Of God, of course. Take care and thanks for watching! :D. From: Luca Zadra Views: 2 0 ratings Time: 05:38 More in Music
            • [APH MMD] Lamb. Axis
              [APH MMD] Lamb. Axis romano,nyo!romano et germany mon beaucoup, beaucoup beaucoup embeter (est sa se voit :( ) twitter:https://twitter.com/yukikuran03 deviantArt:http://mimicraft2503.deviantart.com/ tumblr:https://ww... From: Melina Colin Views: 1 0 ratings Time: 03:56 More in Film & Animation
            • Two hands Mary had a little lamb
              Two hands Mary had a little lamb Kai Xing was trying to play in both hands. From: Wen Jye Chi Views: 0 0 ratings Time: 00:21 More in People & Blogs
            • Let's Play: The Binding of Isaac: Wrath of the Lamb | Glück aber kein können (2/2) [GERMAN HD]
              Let's Play: The Binding of Isaac: Wrath of the Lamb | Glück aber kein können (2/2) [GERMAN HD] Für mehr Informationen Beschreibung öffnen Kostenlos Abonnieren: http://www.youtube.com/subscription_center?add_user=FunnyLetsPlayed Spiel: The Binding of Isaac: Wrath of the Lamb Publisher:. From: FunnyLetsPlayed Views: 0 0 ratings Time: 10:01 More in Gaming
            • Let's Play The Binding of Isaac Wrath of the Lamb #098.1 Die schönen Herzen
              Let's Play The Binding of Isaac Wrath of the Lamb #098.1 Die schönen Herzen Besucht mich auf Facebook: http://on.fb.me/1ucVSM2 □ Ihr wollt kein Let's Play verpassen? http://bit.ly/1kqzTcB □ Ihr Wollt selber auch League of Legends spielen? Dann meldet euch... From: Perrick Views: 267 21 ratings Time: 19:02 More in Gaming
            • The Binding Of Isaac: Wrath Of The Lamb #139 (58. Run)
              The Binding Of Isaac: Wrath Of The Lamb #139 (58. Run) The Binding Of Isaac Playlist: http://bit.ly/1r9UGsl ▻ Videospiele günstig kaufen: http://bit.ly/14J2oiZ ▻ Livestreams: http://www.twitch.tv/wolverous ▻ Let's Play Übersicht: http://bi... From: TheWolverous Views: 0 0 ratings Time: 16:13 More in Gaming
            • The Lamb is worthy - Terry MacAlmon (2010)
              The Lamb is worthy - Terry MacAlmon (2010) This recording was captured during The Heart of Worship Conference 2010 in Zelhem, The Netherlands. This was a conference of Jaweh Ministries International, now known as LoveUnlimited. For... From: LoveUnlimited Views: 6 0 ratings Time: 07:49 More in People & Blogs
            • #003 Lets Play The Binding of Isaac - Wrath of the Lamb "Tod durch Baby. YAY ^.^"
              #003 Lets Play The Binding of Isaac - Wrath of the Lamb "Tod durch Baby. YAY ^.^" Diese Reihe ist eine Vorbereitung auf Rebirth also erwartet keine überkrassen mega moves xD. From: Tharosa LP Views: 0 0 ratings Time: 15:23 More in Gaming
            • Let's Play: The Binding of Isaac: Wrath of the Lamb | Glück aber kein können (1/2) [GERMAN HD]
              Let's Play: The Binding of Isaac: Wrath of the Lamb | Glück aber kein können (1/2) [GERMAN HD] Für mehr Informationen Beschreibung öffnen Kostenlos Abonnieren: http://www.youtube.com/subscription_center?add_user=FunnyLetsPlayed Spiel: The Binding of Isaac: Wrath of the Lamb Publisher:. From: FunnyLetsPlayed Views: 1 0 ratings Time: 09:36 More in Gaming
            • Happy Halloween from Lamb CMC
              Happy Halloween from Lamb CMC Lamb presents the dark side of London... Happy Halloween! From: brandformula . Views: 0 0 ratings Time: 00:27 More in Entertainment
            • [MMD] Lamb.Hatsune Miku
              [MMD] Lamb.Hatsune Miku https://www.facebook.com/duy.huynh.735. From: khanh duy huynh Views: 27 0 ratings Time: 03:59 More in People & Blogs

            •      Related on Wikipedia




























               

              Powered free by PHPmotion