LamB maltoporin of Salmonella typhimurium isolation purification and comparison of sugar binding with LamB of Escherichia coli


     Related Videos
Tracking system is dam good news for salmon
Sea lice may be driving wild salmon to extinction
The Holy Cow and the Lamb of God in Hinduism and Judaism Pt 1
The Holy Cow and the Lamb of God in Hinduism and Judaism Pt 2
Heaven - The Lion and the Lamb - YouTube

     Related Hubpages

  •  Doc. Url:    Embed Code: 

  • PubMed  status
    (0) (0 Votes)
    Views: (1031)   Date: (27-01-09)   Pages: ()
  • Author:  Sch?lein K  Benz R.  

  • Abstract:  LamB (maltoporin) of Salmonella typhimurium: isolation, purification and comparison of sugar binding with LamB of Escherichia coli. Sch?lein K, Benz R. Lehrstuhl f?r Biotechnologie, Universit?t W?rzburg, FRG. LamB (maltoporin) of Salmonella typhimurium was found to be more strongly associated with the murein than OmpF. It was purified in one step using a hydroxyapatite (HTP) column. Reconstitution of the pure protein with lipid bilayer membrane showed that LamB of S. typhimurium formed small ion-permeable channels with a single channel conductance of about 90 pS in 1 M KCl and some preference for cations over anions. The conductance concentration curve was linear, which suggested that LamB of S. typhimurium does not contain any binding site for ions. Pore conductance was completely inhibited by the addition of 20 mM maltotriose. Titration of the LamB-induced membrane conductance with different sugars, including all members of the maltooligosaccharide series up to seven glucose residues, suggested that the channel contains, like LamB (maltoporin) of Escherichia coli, a binding site for sugars. The binding constant of sugars of the maltooligosaccharide series increased with increasing number of glucose residues up to five (saturated). Small sugars had a higher stability constant for sugar binding relative to LamB of E. coli. The advantage of a binding site inside a specific porin for the permeation of solutes is discussed with respect to the properties of a general diffusion porin. PMID: 1693746 [PubMed - indexed for MEDLINE]

     Related Documents

         Related Groups

           Related Science News

             More on Sciencestage

               Answers

                 News
            • Superior Farms Celebrates Lamb With Sacramento Restaurant Contest
              Passionate about lamb and only 20 miles away from the state capital, Superior Farms is sponsoring the Sacramento Farm To Fork festival. In addition to having lamb on the menu for t
            • Discover Nevada: Lamb family's deep Nevada roots
               If there is an equivalent in Nevada to the Kennedy family of Massachusetts, it would probably be the Lambs. During the 60s and 70s, the Lamb family was considered a political dynasty, Nevada's version of royalty.
            • App State loses heartbreaker at Southern Miss, 21-20
              Taylor Lamb threw what appeared to be a game-tying two-yard touchdown pass to Simms McElfresh with six seconds to go but the ensuing extra point was blocked and Appalachian State University football suffered a heartbreaking 21-20 defeat at Southern Miss.
            • Lamb for $5? Think Gaslamp. think Azerbaijan
              Sunset. Goin' with the flow like a pooh stick in a slow crick, sauntering with the crowd down Fifth Avenue. It's the height of happy hour. Conventioneers have been let out and are looking for sunset specials. Lot of Wild West places seem to be doing well.
            • Lamb's slam gives Arizona 5-2 win over Rockies
              Jake Lamb knew Adam Ottavino throws so hard that if he connected at all, the ball could go a long way. Lamb drove Ottavino's 97-mph fastball over the porch in left-center for a grand slam, and the Arizona Diamondbacks rallied to beat the Colorado Rockies 5-2 on Friday night. He got Mark Trumbo to bounce into a fielder's choice that could have been a double play if the reliever was able to get ...
            • Jake Lamb, David Peralta still out for Diamondbacks
              Diamondbacks third baseman Jake Lamb (quad) and outfielder David Peralta (back) remain absent from the lineup Wednesday against visiting San Francisco.
            • Jake Lamb dealing with a quad injury
              Diamondbacks infielder Jake Lamb was held out of the starting lineup Monday night against San Francisco, due to a quad injury, the Arizona Republic reports. He has now missed three consecutive games with the ailment. Lamb is hitting .227 with three homers and 10 RBI over 88 at-bats.
            • Southern Miss beats Appalachian State 21-20
              Dasman McCullum blocked an extra-point attempt with 6 seconds remaining and Southern Miss beat Appalachian State 21-20 Saturday night. ASU's Taylor Lamb threw a 2-yard touchdown pass to Simms McElfresh in the back corner of the end zone to get the Mountaineers within a point. Appalachian State (1-2) tied the game at 14 on a 10-yard touchdown pass from Lamb to Malachi Jones with 5:08 to play.
            • Gaddis' late TD catch stuns Coyotes in wild finish
              Calallen quarterback Gaige Lamb had plenty of time to scope out the Alice defense Friday night. The sophomore signal caller spent the better part of three quarters on the bench for the Wildcats.
            • Rocky Hill Film Producer Gets Rights To Wally Lamb Book
              Synthetic Cinema Branching Out From Horror Films With 'Wishin' And Hopin'' Synthetic Cinema Branching Out From Horror Films With 'Wishin' And Hopin''

                 Related on Youtube Results
          • 【MMD】Lamb 【初音ミク】
            【MMD】Lamb 【初音ミク】 From: 千石撫子 Views: 0 0 ratings Time: 04:02 More in People & Blogs
          • Worthy is the lamb
            Worthy is the lamb Concert organisé à l'occasion de la visite de l'archevêque de douala au Canada. From: frank-lionel djiki Views: 0 0 ratings Time: 02:20 More in Music
          • ALERT: It's Time to Rise! A call to action by Jessica Lamb
            ALERT: It's Time to Rise! A call to action by Jessica Lamb Calling all Patriots! From: Freda Views: 56 8 ratings Time: 08:32 More in Nonprofits & Activism
          • amy lamb when i was 9 months old to 2 years old
            amy lamb when i was 9 months old to 2 years old From: amy lamb Views: 0 0 ratings Time: 03:13 More in People & Blogs
          • Lamb Of God - Redneck
            Lamb Of God - Redneck Original Video : Matt Trakker https://www.youtube.com/watch?v=es6bnABmdsw Paul Dore https://www.youtube.com/watch?v=U-SCrxg-9BE. From: Yannick Dumont Views: 2 0 ratings Time: 03:46 More in People & Blogs
          • 【東方MMD】 Lamb. 【幽香/勇儀】
            【東方MMD】 Lamb. 【幽香/勇儀】 http://www.nicovideo.jp/watch/sm24479839. From: Kamui Tatsugami Views: 17 1 ratings Time: 04:17 More in People & Blogs
          • Christ The Lamb
            Christ The Lamb The combination of a great song and a pictures from a very special movie. From: Brad Roark Views: 0 0 ratings Time: 06:35 More in Howto & Style
          • Great is thy faithfulness/worthey is the lamb
            Great is thy faithfulness/worthey is the lamb Just messing around.....but tell me what you think. From: DSchopz Views: 0 0 ratings Time: 01:33 More in People & Blogs
          • 【APヘタリアMMD】海軍普.争奪Lamb. byアルマダ海賊(改変)
            【APヘタリアMMD】海軍普.争奪Lamb. byアルマダ海賊(改変) 『信じてもどうせ裏切るんだろ?』 前回つべだと画質かなり良くなるんだとなり、コレもちょっと画質悪くて不満だったのでやってみました。... From: kamhana Views: 15 0 ratings Time: 04:10 More in Film & Animation
          • New channel - Lamb Ghost
            New channel - Lamb Ghost From: Slowmoson Views: 0 0 ratings Time: 01:05 More in People & Blogs
          • The Binding of Isaac Wrath of the Lamb#2:AE POAAAAR CONSIGUIMOOOO
            The Binding of Isaac Wrath of the Lamb#2:AE POAAAAR CONSIGUIMOOOO Please Like. From: LDGhostgamer - Views: 1 0 ratings Time: 35:51 More in Gaming
          • 【MMD】Lamb. 【Miku-Nine-Appearance】
            【MMD】Lamb. 【Miku-Nine-Appearance】 From Bilibili.tv Link Download: http://www.miku96.com/998 Please use the translation of Google in the upper right corner. From: hexie hexie Views: 1 0 ratings Time: 04:01 More in Entertainment
          • Worship the Lamb
            Worship the Lamb From: Daniel Gorski Views: 2 0 ratings Time: 04:40 More in Music
          • ARI@COL: Lamb blasts a solo shot to right
            ARI@COL: Lamb blasts a solo shot to right 9/20/14: Jake Lamb hits a line drive that clears the wall in right for a solo home run Check out http://m.mlb.com/video for our full archive of videos, and subscribe on YouTube for the best,... From: MLB Views: 1 0 ratings Time: 00:27 More in Sports
          • 150 15th St W North Vancouver BC V7M1R7 - David Lamb - Sutton West Coast Realty North Vancouver
            150 15th St W North Vancouver BC V7M1R7 - David Lamb - Sutton West Coast Realty North Vancouver http://tours.suttonwestcoast.com/viewer/default.aspx?tourid=937116&refURL=youtube Contact: David Lamb Sutton West Coast Realty North Vancouver 604-868-3414 davidrlamb@shaw.ca for more... From: BCRealEstateTour Views: 0 0 ratings Time: 01:12 More in Travel & Events
          • Lamb Of God - What i've become guitar cover (HD)
            Lamb Of God - What i've become guitar cover (HD) Lamb Of God - what ive become, very nice and powerfull song, i only play the rhythm guitar so i hope you like it and please leave a comment and subscribe if you want more lamb of god covers, thank's. From: LambOfGodRPRS Rippers Views: 3 0 ratings Time: 03:44 More in Music
          • Wee lamb shakes pear
            Wee lamb shakes pear From: Danny Sugar Views: 0 0 ratings Time: 00:58 More in People & Blogs
          • Amy lamb 6 years and younger
            Amy lamb 6 years and younger I created this video with the YouTube Video Editor (http://www.youtube.com/editor) From: amy lamb Views: 0 0 ratings Time: 01:38 More in People & Blogs
          • Lamb and Lion Round 3 Parts
            Lamb and Lion Round 3 Parts Such a pretty round! I wish I had the range to do it in more than one octave. From: Robbybox333 Views: 5 0 ratings Time: 01:17 More in Pets & Animals
          • John Tavener - The Lamb (2003)
            John Tavener - The Lamb (2003) John Tavener's 1982 choral setting of William Blake's "The Lamb," as recorded by Temple Church Choir under the direction of Stephen Layton Little Lamb who made thee Dost thou know... From: possiblyfake Views: 2 0 ratings Time: 03:34 More in Music
          • Mama sheep and Newborn Lamb
            Mama sheep and Newborn Lamb Having a bad day? Here's a cute lil lamb for you. From: Alice Buck Views: 1 0 ratings Time: 00:49 More in People & Blogs
          • Watch The Lamb Part 3 of 4 Taj Placleb
            Watch The Lamb Part 3 of 4 Taj Placleb From: Truth Caption Ministries Views: 0 0 ratings Time: 01:29:52 More in People & Blogs
          • Mary little lamb wierdness
            Mary little lamb wierdness Awesome. From: Filming Crew Supreme Views: 0 0 ratings Time: 00:18 More in Entertainment
          • Let's Play The Binding of Isaac: Wrath of The Lamb Blind 40 - Murdering Eye
            Let's Play The Binding of Isaac: Wrath of The Lamb Blind 40 - Murdering Eye If I had 2 I could do a death glare. From: ZaWizzard Views: 1 0 ratings Time: 16:18 More in Gaming
          • Mary had a little lamb violin
            Mary had a little lamb violin From: Jykeeya Smith Views: 0 0 ratings Time: 01:01 More in People & Blogs

          •      Related on Wikipedia




























               

              Powered free by PHPmotion