LamB maltoporin of Salmonella typhimurium isolation purification and comparison of sugar binding with LamB of Escherichia coli


     Related Videos
Tracking system is dam good news for salmon
Sea lice may be driving wild salmon to extinction
The Holy Cow and the Lamb of God in Hinduism and Judaism Pt 1
The Holy Cow and the Lamb of God in Hinduism and Judaism Pt 2
Heaven - The Lion and the Lamb - YouTube

     Related Hubpages

  •  Doc. Url:    Embed Code: 

  • PubMed  status
    (0) (0 Votes)
    Views: (1031)   Date: (27-01-09)   Pages: ()
  • Author:  Sch?lein K  Benz R.  

  • Abstract:  LamB (maltoporin) of Salmonella typhimurium: isolation, purification and comparison of sugar binding with LamB of Escherichia coli. Sch?lein K, Benz R. Lehrstuhl f?r Biotechnologie, Universit?t W?rzburg, FRG. LamB (maltoporin) of Salmonella typhimurium was found to be more strongly associated with the murein than OmpF. It was purified in one step using a hydroxyapatite (HTP) column. Reconstitution of the pure protein with lipid bilayer membrane showed that LamB of S. typhimurium formed small ion-permeable channels with a single channel conductance of about 90 pS in 1 M KCl and some preference for cations over anions. The conductance concentration curve was linear, which suggested that LamB of S. typhimurium does not contain any binding site for ions. Pore conductance was completely inhibited by the addition of 20 mM maltotriose. Titration of the LamB-induced membrane conductance with different sugars, including all members of the maltooligosaccharide series up to seven glucose residues, suggested that the channel contains, like LamB (maltoporin) of Escherichia coli, a binding site for sugars. The binding constant of sugars of the maltooligosaccharide series increased with increasing number of glucose residues up to five (saturated). Small sugars had a higher stability constant for sugar binding relative to LamB of E. coli. The advantage of a binding site inside a specific porin for the permeation of solutes is discussed with respect to the properties of a general diffusion porin. PMID: 1693746 [PubMed - indexed for MEDLINE]

     Related Documents

         Related Groups

           Related Science News

             More on Sciencestage

               Answers

                 News

                   Related on Youtube Results
            • Techin playing Mary had a little lamb on November 24, 2014
              Techin playing Mary had a little lamb on November 24, 2014 From: Duane Whitcomb Views: 0 0 ratings Time: 01:25 More in Comedy
            • MARY HAD A LITTLE LAMB
              MARY HAD A LITTLE LAMB KREIZTAL HAYLEY... From: joy linga Views: 0 0 ratings Time: 02:26 More in People & Blogs
            • TOP New Ametek Lamb Vacuum Motor 116765-13 including shipping.
              TOP New Ametek Lamb Vacuum Motor 116765-13 including shipping. TOP New Ametek Lamb Vacuum Motor 116765-13 including shipping. http://www.amazon.com/gp/product/B004HLOQJO?tag=hoki07-20. From: Abelino Davide Views: 0 0 ratings Time: 01:46 More in Science & Technology
            • Following The Lamb in Exile ~ Gene Binder
              Following The Lamb in Exile ~ Gene Binder Rabbi Gene concludes the Living in Exile series with a message on what may be one of the most important keys to understanding the story of God: the Lamb. Throughout the scriptures we read about... From: CornerstoneBoulderCO Views: 0 0 ratings Time: 37:45 More in Education
            • The Binding of ISAAC - Wrath Of the lamb / Que noob que soy en esto :v
              The Binding of ISAAC - Wrath Of the lamb / Que noob que soy en esto :v Hola :v. From: Razor_Hawk_361 Views: 1 1 ratings Time: 10:53 More in Gaming
            • Lamb of god - Ruin guitar cover
              Lamb of god - Ruin guitar cover Me playing ruin by lamb of god. I do not own the rights to this song. From: Jake Trammell Views: 0 0 ratings Time: 03:41 More in People & Blogs
            • 踊ってみた LAMB GIRLS【宅舞初投】Dance Cover【GV画质】根本停不下来≖‿≖✧累出shi啦 1
              踊ってみた LAMB GIRLS【宅舞初投】Dance Cover【GV画质】根本停不下来≖‿≖✧累出shi啦 1 ホームメイド( '· )ノバテック( ._ 。 `)あなたの宝物に早期紫色の夜の香りの組み合わせが、 ( 。 •‸• 。 )上海CJが低下に通行料を上げ... From: 我妻由乃 Views: 0 0 ratings Time: 09:00 More in Entertainment
            • Lamb - As Satellites Go By - Live at Rotterdamse Schouwburg
              Lamb - As Satellites Go By - Live at Rotterdamse Schouwburg Lamb - As Satellites Go By Backspace Unwind Tour Live at Rotterdamse Schouwburg, Netherlands 24 November 2014. From: Josef The Views: 1 0 ratings Time: 04:57 More in People & Blogs
            • 2014 Jesus Lamb of God worthy is your name Lion tribe of Judah
              2014 Jesus Lamb of God worthy is your name Lion tribe of Judah 2014 Jesus Lamb of God worthy is your name Lion tribe of Judah The Bible the only truth states John 1:1-16, 29-34 The Eternal Word In the beginning was the W. Jesus says we are the Light... From: Trevor Ford Views: 0 0 ratings Time: 03:34 More in News & Politics
            • Leg of Lamb and Rack of Lamb
              Leg of Lamb and Rack of Lamb Platt College OKC, Ok. From: Jordan White Views: 0 0 ratings Time: 07:23 More in People & Blogs
            • Bottle feeding a Shetland lamb
              Bottle feeding a Shetland lamb Bottle feeding our orphan Shetland lamb. She is now fully grown and doing well. From: Rosie Hazleton Views: 1 0 ratings Time: 00:17 More in People & Blogs
            • In Binary by Lamb 24.11.2014
              In Binary by Lamb 24.11.2014 From: alien species Views: 0 0 ratings Time: 00:47 More in People & Blogs
            • Reign 2x04 Canadian Promo HD The Lamb And The Slaughter Season 2 Episode 4 Canadian Promo
              Reign 2x04 Canadian Promo HD The Lamb And The Slaughter Season 2 Episode 4 Canadian Promo From: Titanic Views: 0 0 ratings Time: 00:31 More in People & Blogs
            • Reign 2x04 Extended Promo HD The Lamb And The Slaughter Season 2 Episode 4 Extended Promo
              Reign 2x04 Extended Promo HD The Lamb And The Slaughter Season 2 Episode 4 Extended Promo From: Titanic Views: 0 0 ratings Time: 00:31 More in People & Blogs
            • Gabriel by Lamb 24.11.2014
              Gabriel by Lamb 24.11.2014 From: alien species Views: 0 0 ratings Time: 00:30 More in People & Blogs
            • Writing Music and Running at State, Kevin Lamb is this week's All-Star Student
              Writing Music and Running at State, Kevin Lamb is this week's All-Star Student Between running in cross country, writing his own original music, and performing in band & choir, WJON's All-Star Student of the Week still finds time to be a high school student. From: AM 1240 WJON Views: 2 0 ratings Time: 02:01 More in Film & Animation
            • Redrum Six play Mary Had a Little Lamb (Stevie Ray Vaughan)
              Redrum Six play Mary Had a Little Lamb (Stevie Ray Vaughan) Redrum Six, cover band milanese: - Eleonora (voce, chitarra acustica) - Maurizio (voce) - Franz (tastiere) - Antonio (chitarra) - Flavio (basso) - Claudio (batteria) From: galfla Views: 0 0 ratings Time: 04:12 More in Music
            • Roberto & the Tickety2 - Mutton dressed as lamb - live @ Inchyra Arts Club
              Roberto & the Tickety2 - Mutton dressed as lamb - live @ Inchyra Arts Club Look out your speedos and come join us at www.robertocassani.co.uk. From: rivoltano Views: 0 0 ratings Time: 03:04 More in Music
            • Rich Aucoin - Behold the Lamb - Personnal Publication - 2008
              Rich Aucoin - Behold the Lamb - Personnal Publication - 2008 http://www.richaucoin.ca/ http://richaucoin.bandcamp.com/ https://www.facebook.com/richaucoinmusic https://now.jbhifi.com.au/Album/837101289245. From: bonzoboydog Views: 0 0 ratings Time: 06:00 More in Music
            • 2013 LAKE CITY CONFERENCE: SONG OF THE LAMB by Mishkanim (Aline & Howie)
              2013 LAKE CITY CONFERENCE: SONG OF THE LAMB by Mishkanim (Aline & Howie) Description. From: LOVEROFYHVH777 Views: 0 0 ratings Time: 08:22 More in Education
            • ??? vs The Lamb - The Binding of Isaac Rebirth
              ??? vs The Lamb - The Binding of Isaac Rebirth From: icogetch Views: 0 0 ratings Time: 02:00 More in Entertainment
            • Lamb Of God - Redneck (Live feat. Paweł "Pavulon" Jaroszewicz)
              Lamb Of God - Redneck (Live feat. Paweł "Pavulon" Jaroszewicz) This footage was filmed during Decapitated's last show on tour with Lamb of God in the US in May/June 2013. I'd like to thank everybody involved: Chris, Willie, Mark, John and Randy for... From: Paweł "Pavulon" Jaroszewicz Views: 3 0 ratings Time: 04:47 More in Music
            • Drawdio- Mary had a little lamb
              Drawdio- Mary had a little lamb From: MsKroesen Views: 0 0 ratings Time: 00:15 More in Comedy
            • Abbie plays "Mary Had a Little Lamb" Nov.24, 2014
              Abbie plays "Mary Had a Little Lamb" Nov.24, 2014 Abbie plays "Mary Had a Little Lamb" Nov.24, 2014. From: Susan Hung Views: 0 0 ratings Time: 00:16 More in Music
            • Mary Had A Little Lamb - Nursery Rhymes
              Mary Had A Little Lamb - Nursery Rhymes Mary Had A Little Lamb - Nursery Rhymes. From: Maxi Comptines Views: 0 0 ratings Time: 00:59 More in Film & Animation

            •      Related on Wikipedia




























               

              Powered free by PHPmotion