Bovine viral diarrhea virus contamination of nutrient serum cell cultures and viral vaccines


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    • Author:  Levings RL  Wessman SJ.  

    • Abstract:  Bovine viral diarrhea virus contamination of nutrient serum, cell cultures and viral vaccines. Levings RL, Wessman SJ. National Veterinary Services Laboratories, Animal and Plant Health Inspection Service, USDA, Ames, IA 50010. Bovine viral diarrhea virus (BVDV) infection is common in the bovine population. Infection in utero leads to virus and antibody contamination of the fetal bovine serum used in cell cultures. These contaminants can interfere with diagnosis of viral infection. The high frequency of virus and antibody detection in individual animal or small pool samples suggests that any large pool of unscreened sera will be contaminated. Infection of cell cultures with BVDV can lead to interference with the growth of other viruses. Vaccine produced on contaminated cells may in turn be contaminated, leading to seroconversion or disease in the vaccine. The safety, purity, and efficacy of viral vaccines require BVDV testing of ingredients, cell substrates and final product. Methods for detection of BVDV in nutrient serum, cell cultures, seed viruses, and viral vaccines, and the frequency of their detection at the National Veterinary Services Laboratories are discussed. PMID: 1665461 [PubMed - indexed for MEDLINE]

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