Microbeam laser-injured neurons increase in vitro astrocytic gap junctional communication as measured by fluorescence recovery after laser photobleaching

Microbeam laser-injured neurons increase in vitro astrocytic gap junctional communication as measured by fluorescence recovery after laser photobleaching. Anders JJ, Woolery S. Department of Anatomy and Cell Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814. An important aspect of the neuronal-astrocyte relationship is the interaction of reactive astrocytes with injured and/or dying neurons. Few studies have focused on the signaling of astrocytes by injured neurons or on the possibility that neurons can ...

Abstract A design is given of a laser ion source for the 4.2 MV Van de Graaff accelerator at the Columbia University Radiological Research Accelerator Facility (RARAF). The source has been designed with application in mind for the RARAF single-particle single-cell microbeam, though it will also be used for broad-beam irradiations. The operating principle, laser ablation, can produce heavy ions with high charge states so that their energies will be high enough to provide sufficient range-at least 20 ?m-for irradiating cells on a thin surface at...

Abstract A design is given of a laser ion source for the 4.2 MV Van de Graaff accelerator at the Columbia University Radiological Research Accelerator Facility (RARAF). The source has been designed with application in mind for the RARAF single-particle single-cell microbeam, though it will also be used for broad-beam irradiations. The operating principle, laser ablation, can produce heavy ions with high charge states so that their energies will be high enough to provide sufficient range-at least 20 ?m-for irradiating cells on a thin surface at...

Manipulation of cells, organelles, and genomes by laser microbeam and optical trap. Weber G, Greulich KO. Max-Planck-Institut f?r Zellbiologie, Ladenburg, Germany. PMID: 1577585 [PubMed - indexed for MEDLINE]

Abstract The decomposition mechanism of hard a-C films under irradiation using a microbeam laser (514.5 nm) was studied. The films were magnetron sputtered at different pressures to achieve different degrees of sp3/sp2 bonding ratio. Under laser irradiation, they showed graphitization and decomposition. The graphitization saturated at early exposure of ?4 min, while the decomposition progressed exponentially with the exposure time, following a first-order reaction mechanism. The decomposition of a-C films under laser irradiation can be modeled...

Abstract The decomposition mechanism of hard a-C films under irradiation using a microbeam laser (514.5 nm) was studied. The films were magnetron sputtered at different pressures to achieve different degrees of sp3/sp2 bonding ratio. Under laser irradiation, they showed graphitization and decomposition. The graphitization saturated at early exposure of ?4 min, while the decomposition progressed exponentially with the exposure time, following a first-order reaction mechanism. The decomposition of a-C films under laser irradiation can be modeled...

Abstract The decomposition mechanism of hard a-C films under irradiation using a microbeam laser (514.5 nm) was studied. The films were magnetron sputtered at different pressures to achieve different degrees of sp3/sp2 bonding ratio. Under laser irradiation, they showed graphitization and decomposition. The graphitization saturated at early exposure of ?4 min, while the decomposition progressed exponentially with the exposure time, following a first-order reaction mechanism. The decomposition of a-C films under laser irradiation can be modeled...

Abstract Experimental laser microbeam techniques have become established tools for studying living specimens. A steerable, focused laser beam may be used for a variety of experimental manipulations such as laser microsurgery, optical trapping, localized photolysis of caged bioactive probes, and patterned photobleaching. Typically, purpose-designed experimental systems have been constructed for each of these applications. In order to assess the consequences of such experimental optical interventions, long-term, microscopic observation of the sp...

Abstract Experimental laser microbeam techniques have become established tools for studying living specimens. A steerable, focused laser beam may be used for a variety of experimental manipulations such as laser microsurgery, optical trapping, localized photolysis of caged bioactive probes, and patterned photobleaching. Typically, purpose-designed experimental systems have been constructed for each of these applications. In order to assess the consequences of such experimental optical interventions, long-term, microscopic observation of the sp...

Abstract Generally good agreement is obtained between the single-event output voltage transient waveforms obtained by exposing individual circuit elements of a bipolar comparator and operational amplifier to an ion microbeam, a pulsed laser beam, and circuit simulations using SPICE. The agreement is achieved by adjusting the amounts of charge deposited by the laser or injected in the SPICE simulations. The implications for radiation hardness assurance are discussed.

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