Rejuvenation of erythrocytes preserved with AS-1 and AS-3
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PubMed
(0) (0 Votes)Views: (1051) Date: (20-01-09) Pages: () -
Author: Brecher ME Zylstra-Halling VW Pineda AA.
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Abstract: Rejuvenation of erythrocytes preserved with AS-1 and AS-3. Brecher ME, Zylstra-Halling VW, Pineda AA. Section of Transfusion Medicine, Mayo Clinic, Rochester, Minnesota 55905. Rejuvenation of erythrocytes (biochemical modification of adenosine triphosphate and 2,3-diphosphoglycera te) preserved with currently available nutrient additive solutions has not been licensed or adequately studied. The ability of the commercially available solution PIPA to modify erythrocytes preserved with AS-1 or AS-3 for 35 and 42 days was studied. Use of PIPA produced greater-than-normal levels of adenosine triphosphate in the preserved erythrocytes. Restoration of 2,3-diphosphoglycera te was suboptimal at both periods. (PIPA treatment produces normal levels of 2,3-diphosphoglycera te in erythrocytes preserved for 35 days with citrate-phosphate-de xtrose-adenine-1.) In erythrocytes preserved with AS-1 or AS-3 for 42 days, 2,3-diphosphoglycera te returned to normal levels after a second PIPA rejuvenation. This study demonstrates that biochemical modification is possible in erythrocytes preserved with AS-1 or AS-3 for 42 days. PMID: 1746494 [PubMed - indexed for MEDLINE]
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