Isolation of a rat S100 alpha cDNA and distribution of its mRNA in rat tissues
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Isolation Methods Used in Oper...
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Use of the Erickson Vac-Ejector, suedapter and cotton roll holders. These methods are used during pr...
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Safety Micropipetter Microscop...
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UC Berkeley, General Biology Laboratory: Safety, Micropipetter, Microscope, Cells and Vibrio isolati...
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PubMed
(0) (0 Votes)Views: (1010) Date: (20-01-09) Pages: () -
Author: Zimmer DB Song W Zimmer WE.
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Abstract: Isolation of a rat S100 alpha cDNA and distribution of its mRNA in rat tissues. Zimmer DB, Song W, Zimmer WE. Department of Pharmacology, University of South Alabama School of Medicine, Mobile 36688. In order to clarify the reported discrepancies in S100 alpha protein and mRNA distribution in rat tissues, a rat S100 alpha cDNA has been isolated and this species homologous probe along with a rat S100 beta cDNA probe has been used to examine S100 mRNA expression in rat tissues. Although the rat S100 alpha cDNA was missing approximately 30 nucleotides of coding sequence, only 4 conservative changes in amino acid sequence were observed when the deduced amino acid sequence was compared to the bovine S100 alpha amino acid sequence. Thus, S100 alpha proteins, like S100 beta proteins, are highly conserved among species. All nineteen of the tissues examined (including cerebrum and cerebellum) contained S100 alpha mRNA. In addition, S100 beta mRNA was detected in thirteen of the nineteen tissues examined. These results are in agreement with previous protein distribution studies and further demonstrate that S100 proteins are not brain-specific and are expressed in a large number of tissues. Although S100 alpha and S100 beta mRNAs were detected in rat tissues which had previously been reported to contain S100 alpha and S100 beta protein, a direct correlation between the protein and mRNA levels were not observed, suggesting that different mechanisms regulate S100 expression in various tissues. S100 alpha exhibited a single similar size mRNA species (0.5 Kb) in all tissues examined, as did S100 beta (1.5 Kb), suggesting that the individual S100 proteins are expressed as single mRNA and protein products in rat tissues. PMID: 1742602 [PubMed - indexed for MEDLINE]
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