Generation and characterization of two monoclonal self-associating IgG rheumatoid factors from a rheumatoid synovium

Abstract:  Generation and characterization of two monoclonal self-associating IgG rheumatoid factors from a rheumatoid synovium. Lu EW, Deftos M, Tighe H, Carson DA, Chen PP. Department of Medicine, University of California, San Diego 92093. OBJECTIVE. To study IgG rheumatoid factor (RF) from rheumatoid synovium. METHODS. We fused the K6H6/B5 human-mouse heterohybridoma with unstimulated rheumatoid synovial B cells to generate IgG-RF-secreting hybridomas. RESULTS. The RFs from 2 such hybridomas bound specifically to the Fc fragment of human IgG and self-associated to form immune complexes. Such immune complexes are a major characteristic of the pathogenic IgG-RFs in rheumatoid synovium. CONCLUSION. IgG-RF-secreting hybridomas have been obtained. Analyses may reveal the underlying mechanisms of the induction of IgG-RF. PMID: 1731807 [PubMed - indexed for MEDLINE]