DAPI as a useful stain for nuclear quantitation
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PubMed
(0) (0 Votes)Views: (1066) Date: (20-01-09) Pages: () -
Author: Tarnowski BI Spinale FG Nicholson JH.
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Abstract: DAPI as a useful stain for nuclear quantitation. Tarno wski BI, Spinale FG, Nicholson JH. Department of Pathology, Medical University of South Carolina, Charleston 29425. A simple-to-use fluorescent stain, 4',6-diamidino-2-phe nylindole (DAPI), visualizes nuclear DNA in both living and fixed cells. DAPI staining was used to determine the number of nuclei and to assess gross cell morphology. Following light microscopic analyses, the stained cells were processed for electron microscopy. Cells stained with DAPI showed no ultrastructural changes compared to the appearance of cells not stained with DAPI. DAPI staining allows multiple use of cells eliminating the need for duplicate samples. PMID: 1725854 [PubMed - indexed for MEDLINE]
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