AACR 2009: Preclinical Analysis of Combined PI3K and MEK Inhibition as a Strategy for the Treatment ...
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Abstract: Abstract Increased QRS and ST-T micro-variability and ventricular late potentials are associated with an increased risk for malignant arrhythmias. However, the diagnostic power of the singular parameters is limited. In this study, we investigated the diagnostic ability of a linear combination of five (both variant and static) high-resolution ECG parameters. Continuous and signal-averaged ECGs were recorded from 51 healthy volunteers without any structural heart disease and with no cardiac risk factors and from 44 patients with coronary heart disease and ventricular arrhythmias. Beat-to-beat micro-variability measurement of the QRS complex and the ST-T segment was based on 250 consecutive sinus beats per individual. Signal-averaged ECGs were analyzed with the Simson method. The diagnostic utility of the five singular parameters and all combinations of variables were evaluated by linear discriminant analysis, maximizing the area under the receiver operator characteristic (ROC) curve. We conclude that the combination of de- and re-polarization variability with the static QRS duration markedly improves the detection of patients with inducible ventricular tachycardia (VT)
Three methods were used to combine measurements from biplane coronary views to assess restenosis following PTCA. The first 153 patients from the EMPAR trial with 118 measurable coronary stenoses were chosen for this study. The mean difference between the RAO and LAO projections used was 88 +/- 22 degrees (S.D.). There was no systematic difference in coronary dimensions between RAO and LAO views for the group. Coronary restenosis was primarily defined as loss of 50% or more of the luminal diameter gain from the procedure. The ratio of luminal lo...
BACKGROUND: The Codon Adaptation Index (CAI) was first developed to measure the synonymous codon usage bias for a DNA or RNA sequence. The CAI quantifies the similarity between the synonymous codon usage of a gene and the synonymous codon frequency of a reference set. RESULTS: We describe here CAIcal, a web-server available at http://genomes.urv.es/CAIcal that includes a complete set of utilities related with the CAI. The server provides useful important features, such as the calculation and graphical representation of the CAI along either an i...
OBJECTIVE: To establish a hepatocyte immunotoxicity model for screening of liver protective medications. METHODS: Cytotoxicity was induced by coincubating BCG-pretreated rat hepatocytes in vivo and with 10 mg/L LPS in vitro. Biphenyldimethylesterate (DDB), malotilate(MLT), silybin(SB) and glycyrrhizin (GRZ) were coincubated along with LPS to prevent the hepatocyte injury and verify the applicability and reliability of the model. AST, LDH and nitric oxide (NO) were measured in both the serum and supernatant. The liver and spleen index were calcu...
The combined use of fluorescence microscopy and micromanipulation provides a powerful approach for understanding the mechanochemistry of cell membranes. Fluorescent labeling of erythrocytes has been used to identify particular populations of cells to assess the effects of abnormal deformability on cell survival. It was found that cells deprived of surface area are either eliminated rapidly from the circulation or undergo a reduction in volume to improve cellular deformability. Fluorescence microscopy can also be used to assess the distribution ...
Carp (Cyprinus carpio) and barbel (Barbus graellsii) were collected from five sites along the Ebro River. The study was designed to assess levels of persistent organic pollutants and metals bioaccumulated by fish, and some biochemical responses (cytochrome P450 system, phase II activities, and metallothioneins) against those pollutants. The highest levels of PCBs and DDTs were detected in carp from industrialised areas, which also showed high levels of mercury and cadmium in the liver, and high levels of nonylphenol in bile. Significant alterat...
Ingestion of starch from corn (naturally enriched with (13)C) should produce (13)CO(2) after small intestinal digestion and (13)CO(2) and H(2) from colonic fermentation. This study used a combined (13)CO(2)/H(2) breath test to assess the digestion and fermentation of resistant starch and to show that the test could detect changes in digestibility due to cooking. Volunteers consumed 40 g digestible cornstarch with water (n = 8), or 40 g resistant cornstarch in liquid (n = 12) or cooked into a pancake (n = 4). Interval breath sampling was perform...
BACKGROUND AND OBJECTIVES: Changes in the symptoms of cough after treatment with a combined herbal preparation containing dry ivy leaf extract as main active ingredient, decoction of thyme and aniseed, and mucilage of marshmallow root (Weleda Hustenelixier, new formulation) and its tolerability were investigated in an open clinical trial ('Anwendungsbeleg'). PATIENTS AND METHODS: Between January and March 2004, 13 general practitioners recruited and treated 62 patients with a mean age of 50 years (range 16-89). The patients had irritating cough...
Community-based surveys of iron deficiency (ID) require simple, accurate methods that can be used in remote areas. The objective of this study was to assess iron status in rural Kenya using "field-friendly" methods for capillary blood, including an improved dried blood spot assay for transferrin receptor (TfR). A single finger stick was used to obtain capillary blood from 275 school-age children. Whole blood was applied directly to filter paper, dried, and later analyzed for TfR, as well as C-reactive protein (CRP), an acute-phase protein that ...
AIMS: To assess the significance of combined neurophysiological and neurourological examinations for diagnosis of neurogenic male sexual dysfunction. METHODS: This is a prospective study of 32 spinal cord injured men. Each underwent clinical and neurophysiological examinations (sympathetic skin responses (SSR), pudendal somato-sensory evoked potentials (P-SSEP), bulbocavernosus reflex (BCR)) and neuro-urological measurements (urodynamic examination (UE), reflex erections (RE), psychogenic erections (PE) and nocturnal penile tumescence recording...
Carps (Cyprinus carpio) and red swamp crayfish (Procambarus clarkii) were sampled from two lowstream Mediterranean rivers (Anoia and Cardener) receiving extensive urban and industrial waste water discharges. Tissue residues of selected pollutants (organochlorinated compounds, polycyclic aromatic hydrocarbons (PAHs)) were determined in conjunction with different biochemical responses (cytochrome P450, phase II enzymes) with the aim of investigating whether resident organisms were responsive to changes in water quality. Biota inhabiting those riv...
Gene expression changes in pathophysiological states can be spatiotemporally monitored by in situ hybridization and reliably quantified by real-time RT-PCR. Here we developed a new method whereby adjacent slides of frozen sections can be used for gene expression analysis by in situ hybridization and real-time RT-PCR. We applied this method to assess the mRNA expression of connexin 43 (Cx43), the major astrocytic connexin, after kainate-induced seizures in rat hippocampus. Gap junction-building connexins play a role in the pathogenesis of severa...