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Abstract: The antibacterial activities of essential oils from leaves of two Eucalyptus species (globulus and camaldulensis) was determined against Staphylococcus aureus Gram (+) and Escherichia coli Gram (-) bacteria. The inhibiting activity was evaluated by three methods: aromatogramme, microatmosphere and germs in suspension. Results demonstrated of the leaf essential oils of the two species showed an excellent inhibitory effect on S. aureus than that of E. coli. These data would indicate the potential usefulness of the two Eucalyptus species as a microbiostatic, antiseptic or as disinfectant agent. Citation: Bachir Raho Ghalem and Benali Mohamed; African Journal of Pharmacy and Pharmacology Vol.2(10), pp. 211-215 December,2008.