Double antibody enzyme immunoassay for the quantitation of adenosine 3 , 5 -cyclic monophosphate (cyclic AMP) and guanosine 3 , 5 -cyclic monophosphate (cyclic GMP) in tissue and plasma.
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3D Medical Animation Antibody ...
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www.nucleusinc.com This 3D medical animation shows how antibodies stop harmful pathogens from attach...
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Lecture - 11 Enzyme Mechanisms...
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Lecture Series on biochemistry I by Prof.S.Dasgupta, Dept of Chemistry, IIT Kharagpur. For more deta...
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Lecture - 10 Enzyme Mechanisms...
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Lecture Series on biochemistry I by Prof.S.Dasgupta, Dept of Chemistry, IIT Kharagpur. For more deta...
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Enzyme Catalysis
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This video explains enzyme catalysis with an analogy. This video is from: Essential Cell Biology, 3r...
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Lecture - 28 Enzyme Technology...
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Lecture Series on Enzyme Science and Engineering by Prof.Subhash Chand, Department of Biochemical En...
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PubMed
(0) (0 Votes)Views: (2) Date: (1982-1-1) Pages: () -
Author: M Fujimoto
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Abstract: A sensitive double antibody enzyme immunoassay for the quantitation of cyclic AMP and cyclic GMP is presented. Specific antisera to each nucleotide were raised in rabbits by immunization with succinyl cyclic nucleotide-human serum albumin conjugates. For competitive reaction, antibodies were incubated with a mixture of succinyl cyclic nucleotide labelled with beta-D-galactosidase and unlabelled succinylated standard or sample cyclic nucleotides. The antibody-bound enzyme-hapten was separated from free hapten by anti-rabbit IgG immobilized to a polystyrene ball. Activity of the enzyme on the solid phase was fluorometrically determined. The assay system made it possible to ascertain values as low as 5 fmole of cyclic AMP or cyclic GMP. Cyclic nucleotides in plasma could be accurately determined by this method without requiring a deproteinizing reagent as the first step of assay.
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